DTWD1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1D2]

CAT#: TA811576AM

DTWD1 mouse monoclonal antibody,clone OTI1D2, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DTWD1 (RC227602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DTWD1 (1:2000). Positive lysates LY428603 (100ug) and LC428603 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from U251 cell line by using anti-DTWD1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DTWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811576) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-DTWD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811576) (1:500)

Specifications

Product Data

• Clone Name: OTI1D2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DTWD1 (NP_001138427) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DTW domain containing 1
• Database Link:
  NP_001138427
  Entrez Gene 56986 Human
  UniProt ID Q8N5C7
• Synonyms: MDS009