TOPBP1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5C12]

CAT#: TA811658AM

TOPBP1 mouse monoclonal antibody,clone OTI5C12, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOPBP1 (RC218584, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOPBP. Positive lysates LY416246 (100ug) and LC416246 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5C12
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 892-1146 of human TOPBP1 (NP_008958) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 171.1 kDa
• Gene Name: topoisomerase (DNA) II binding protein 1
• Database Link:
  NP_008958
  Entrez Gene 11073 Human
  UniProt ID Q92547
• Background: This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands. [provided by RefSeq, Jul 2008]
• Synonyms: TOP2BP1