EEF1D Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6B3]

CAT#: TA811677AM

EEF1D mouse monoclonal antibody,clone OTI6B3, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EEF1D (RC224856, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EEF1D (1:2000). Positive lysates LY429804 (100ug) and LC429804 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-EEF1D monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-EEF1D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811677) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-EEF1D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811677) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-EEF1D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811677) (1:2000)

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Specifications

Product Data

• Clone Name: OTI6B3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EEF1D (NP_115754) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: eukaryotic translation elongation factor 1 delta
• Database Link:
  NP_115754
  Entrez Gene 1936 Human
  UniProt ID P29692
• Background: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19. [provided by RefSeq, Aug 2010]
• Synonyms: EF-1D; EF1D; FP1047