CENPJ Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1E3]

CAT#: TA811853BM

CENPJ mouse monoclonal antibody,clone OTI1E3, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CENPJ (RC215682, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CENPJ. Positive lysates LY402685 (100ug) and LC402685 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1E3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1087-1338 of human CENPJ (NP_060921) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 152.8 kDa
• Gene Name: centromere protein J
• Database Link:
  NP_060921
  Entrez Gene 55835 Human
  UniProt ID Q9HC77
• Background: This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
• Synonyms: BM032; CENP-J; CPAP; LAP; LIP1; MCPH6; Sas-4; SASS4; SCKL4