MED9 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7F11]

CAT#: TA811891BM

MED9 mouse monoclonal antibody,clone OTI7F11, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED9 (RC211226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED9. Positive lysates LY413384 (100ug) and LC413384 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MED9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811891) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MED9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811891) (1:1000)

Specifications

Product Data

• Clone Name: OTI7F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MED9 (NP_060489) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.2 kDa
• Gene Name: mediator complex subunit 9
• Database Link:
  NP_060489
  Entrez Gene 55090 Human
  UniProt ID Q9NWA0
• Background: The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
• Synonyms: MED25