LAMP1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI10E1]

CAT#: TA811975BM

LAMP1 mouse monoclonal antibody,clone OTI10E1, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAMP1 (RC219208, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAMP1. Positive lysates LY417223 (100ug) and LC417223 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 cell lines by using anti-LAMP1 monoclonal antibody (1:500).

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  HEK293T cells transfected with either RC219208 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LAMP1 antibody (TA811975), and then analyzed by flow cytometry (1:100).

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  Flow cytometric Analysis of living A431 cells, using anti-LAMP1 antibody (TA811975), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:25).

Specifications

Product Data

• Clone Name: OTI10E1
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:25~100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 29-382 of human LAMP1 (NP_005552) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.88 kDa
• Gene Name: lysosomal associated membrane protein 1
• Database Link:
  NP_005552
  Entrez Gene 3916 Human
  UniProt ID P11279
• Background: The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq, Jul 2008]
• Synonyms: CD107a; LAMPA; LGP120

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Lysosome