Chromogranin C (SCG2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3F12]

CAT#: TA811982AM

SCG2 mouse monoclonal antibody,clone OTI3F12, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCG2 (RC205382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCG2. Positive lysates LY418654 (100ug) and LC418654 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-SCG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811982) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SCG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811982) (1:500)

Specifications

Product Data

• Clone Name: OTI3F12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31 – 617 of human SCG2 (NP_003460) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.8 kDa
• Gene Name: secretogranin II
• Database Link:
  NP_003460
  Entrez Gene 7857 Human
  UniProt ID P13521
• Background: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown. [provided by RefSeq, Jul 2008]
• Synonyms: CHGC; EM66; SgII; SN

Reference Data

• Protein Families: Secreted Protein, Transmembrane