Drebrin (DBN1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4B1]

CAT#: TA812128BM

DBN1 mouse monoclonal antibody,clone OTI4B1, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBN1 (RC200372, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBN1. Positive lysates LY418021 (100ug) and LC418021 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from SHSY-5Y cell line by using anti-DBN1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-DBN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812128) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-DBN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812128) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-DBN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812128) (1:2000)

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Specifications

Product Data

• Clone Name: OTI4B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-240 of human DBN1 (NP_004386) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: drebrin 1
• Database Link:
  NP_004386
  Entrez Gene 56320 MouseEntrez Gene 1627 Human
  UniProt ID Q16643
• Synonyms: D0S117E