Arp3 (ACTR3) Mouse Monoclonal Antibody [Clone ID: OTI5G4]

CAT#: TA812199S

ACTR3 mouse monoclonal antibody,clone OTI5G4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTR3 (RC208460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTR3. Positive lysates LY417113 (100ug) and LC417113 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines by using anti-ACTR3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ACTR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812199) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ACTR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812199) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-ACTR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812199) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5G4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-418 of human ACTR3 (NP_005712) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.2 kDa
• Gene Name: ARP3 actin-related protein 3 homolog (yeast)
• Database Link:
  NP_005712
  Entrez Gene 74117 MouseEntrez Gene 81732 RatEntrez Gene 10096 Human
  UniProt ID P61158
• Background: The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
• Synonyms: ARP3

Reference Data

• Protein Families: Druggable Genome