B7-1 (CD80) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1G9]

CAT#: TA812243AM

CD80 mouse monoclonal antibody,clone OTI1G9, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (RC206540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80 (1:500).

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  Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-CD80 monoclonal antibody (1:500).

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  CD80 ELISA with 1G9 (TA812243) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

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  HEK293T cells transfected with either RC206540 overexpress plasmid (Red) or IgG isotype control (Blue) were immunostained by anti-CD80 antibody (TA812243), and then analyzed by flow cytometry (1:100).

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  Flow cytometric Analysis of living Jurkat cells, using anti-CD80 antibody (TA812243), (Red), compared to an IgG isotype control, (Blue) (1:100).

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  Flow cytometric Analysis of living Raji cells, using anti-CD80 antibody (TA812243), (Red), compared to an IgG isotype control, (Blue) (1:100).

• CD80 Luminex with 1G9 (TA812243) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

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Specifications

Product Data

• Clone Name: OTI1G9
• Applications: ELISA, FC, LMNX, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD80 molecule
• Database Link:
  NP_005182
  Entrez Gene 941 Human
  UniProt ID P33681
• Synonyms: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis