Mutarotase (GALM) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4F4]

CAT#: TA812300BM

GALM mouse monoclonal antibody,clone OTI4F4, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GALM (RC200995, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GALM. Positive lysates LY408487 (100ug) and LC408487 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-GALM monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-GALM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA812300) (1:2000)

Specifications

Product Data

• Clone Name: OTI4F4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GALM (NP_620156) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.6 kDa
• Gene Name: galactose mutarotase
• Database Link:
  NP_620156
  Entrez Gene 313843 RatEntrez Gene 319625 MouseEntrez Gene 130589 Human
  UniProt ID Q96C23
• Background: This gene encodes an enzyme that catalyzes the epimerization of hexose sugars such as glucose and galactose. The encoded protein is expressed in the cytoplasm and has a preference for galactose. The encoded protein may be required for normal galactose metabolism by maintaining the equilibrium of alpha and beta anomers of galactose. [provided by RefSeq, Mar 2009]
• Synonyms: BLOCK25; GLAT; HEL-S-63p; IBD1

Reference Data

• Protein Pathways: Glycolysis / Gluconeogenesis