CD27 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4E4]

CAT#: TA812552BM

CD27 mouse monoclonal antibody,clone OTI4E4, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD27 (RC204252, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD27 (1:500).

• 

  Flow cytometric analysis of living 293T cells transfected with CD27 overexpression plasmid (RC204252), Red)/empty vector (PS100001, Blue) using anti-CD27 antibody (TA812552). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• 

  Flow cytometric analysis of living Raji cells, using anti-CD27 antibody (TA812552, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

• 

  Flow cytometric analysis of living Ramos cells, using anti-CD27 antibody (TA812552, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

• 

  Flow cytometric analysis of living PBMCs, using anti-CD27 antibody (TA812552, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

Show all 5 images

Specifications

Product Data

• Clone Name: OTI4E4
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD27 (NP_001233) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.16 kDa
• Gene Name: CD27 molecule
• Database Link:
  NP_001233
  Entrez Gene 939 Human
  UniProt ID P26842
• Background: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProtKB/Swiss-Prot Function]
• Synonyms: S152; S152. LPFS2; T14; TNFRSF7; Tp55

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction