TIGIT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5G1]

CAT#: TA812567BM

TIGIT mouse monoclonal antibody,clone OTI5G1, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIGIT (RC221447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIGIT (1:1000).

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  Flow cytometric analysis of living 293T cells transfected with TIGIT overexpression plasmid (RC221447), Red)/empty vector (PS100001, Blue) using anti-TIGIT antibody (TA812567). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI5G1
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TIGIT (NP_776160) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: T-cell immunoreceptor with Ig and ITIM domains
• Database Link:
  NP_776160
  Entrez Gene 201633 Human
  UniProt ID Q495A1
• Background: This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [provided by RefSeq, Sep 2009]
• Synonyms: VSIG9; VSTM3; WUCAM

Reference Data

• Protein Families: Transmembrane