CD272 (BTLA) Mouse Monoclonal Antibody [Clone ID: OTI9A10]

CAT#: TA812582S

BTLA mouse monoclonal antibody,clone OTI9A10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA (RC219458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTLA. Positive lysates LY403628 (100ug) and LC403628 (20ug) can be purchased separately from OriGene.

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  Flow cytometric analysis of living 293T cells transfected with BTLA overexpression plasmid (RC219458), Red)/empty vector (PS100001, Blue) using anti-BTLA antibody (TA812582). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI9A10
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31-157+Mouse Fc of human BTLA (NP_861445) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: B and T lymphocyte associated
• Database Link:
  NP_861445
  Entrez Gene 151888 Human
  UniProt ID Q7Z6A9
• Background: This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
• Synonyms: BTLA1; CD272

Reference Data

• Protein Families: Transmembrane