NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: TA890002

Rabbit polyclonal RELA Antibody

Product Images

Western blot analysis of HEK293 cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution.

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  Western blot analysis of HeLa cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution.

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  Western blot analysis of HT29 cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution

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  Western blot analysis of MCF7 cell lysate (35ug) by using Rabbit polyclonal anti-RELA antibody at 1:2000 dilution.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RELA-Knockout HeLa cells (KO, Cat# LC810201) were separated by SDS-PAGE and immunoblotted with anti-RELA monoclonal antibody TA890002, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using Rabbit polyclonal anti-RELA antibody at (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

• Immunohistochemical staining of paraffin-embedded Human adult heart tissue using Rabbit polyclonal anti-RELA antibody at (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

• Immunohistochemical staining of paraffin-embedded Human melanoma tissue using Rabbit polyclonal anti-RELA antibody at (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

• Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using Rabbit polyclonal anti-RELA antibody at (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide conjugated to KLH derived from within residues 100 - 170 of Human RELA.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60 kDa
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  NP_068810
  Entrez Gene 19697 MouseEntrez Gene 309165 RatEntrez Gene 5970 Human
  UniProt ID Q04206
• Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
• Synonyms: NFKB3; p65

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway