beta Actin (ACTB) Rabbit Polyclonal Antibody

CAT#: TA890010

Rabbit polyclonal anti-ACTB(beta Actin) antibody, Loading control

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTB (RC203643, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polyc. Positive lysates LY420311 (100ug) and LC420311 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-ACTB Polyclonal antibody (1:1000).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB 1:1000
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: ACTB Synthetic peptide conjugated to KLH derived from within residues 30-100 of Human ACTB
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.6 kDa
• Gene Name: actin, beta
• Database Link:
  NP_001092
  Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 403580 DogEntrez Gene 574285 MonkeyEntrez Gene 60 Human
  UniProt ID P60709
• Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]
• Synonyms: BRWS1; PS1TP5BP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis