MADH7 (SMAD7) Rabbit Polyclonal Antibody

CAT#: TA890026

Rabbit polyclonal anti-SMAD7 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SMAD7 (Cat# RC210400, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD7 antibody (Cat# TA890026). Positive lysates LY416991 (100ug) and LC416991 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from different cell lines or tissues by using anti-SMAD7 rabbit polyclonal antibody (TA890026).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human SMAD7
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 2.57mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.2 kDa
• Gene Name: SMAD family member 7
• Database Link:
  NP_005895
  Entrez Gene 17131 MouseEntrez Gene 81516 RatEntrez Gene 4092 Human
  UniProt ID O15105
• Background: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
• Synonyms: CRCS3; MADH7; MADH8

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway