ZP3 Rabbit Polyclonal Antibody

CAT#: TA890039

Rabbit polyclonal anti-ZP3 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZP3 (RC210705, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZP3 rabbi. Positive lysates LY429335 (100ug) and LC429335 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines or tissues by using anti-ZP3 rabbit polyclonal antibody (TA890039).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human ZP3
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 2.26mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.1 kDa
• Gene Name: zona pellucida glycoprotein 3 (sperm receptor)
• Database Link:
  NP_009086
  Entrez Gene 7784 Human
  UniProt ID P21754
• Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008]
• Synonyms: Zp-3; ZP3A; ZP3B; ZPC

Reference Data

• Protein Families: Secreted Protein, Transmembrane