INCENP Rabbit Polyclonal Antibody

CAT#: TA890129

Rabbit Polyclonal anti-INCENP Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY INCENP (RC212647, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-INCEN. Positive lysates LY420743 (100ug) and LC420743 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines and tissues by using anti-INCENP rabbit polyclonal antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human INCENP
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 3.23 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 105.2 kDa
• Gene Name: inner centromere protein
• Database Link:
  NP_001035784
  Entrez Gene 16319 MouseEntrez Gene 293733 RatEntrez Gene 3619 Human
  UniProt ID Q9NQS7
• Background: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]). [supplied by OMIM, Mar 2008]
• Synonyms: FLJ31633; MGC111393

Reference Data

• Protein Families: Druggable Genome