MBD1 Rabbit Polyclonal Antibody

CAT#: TA890133

Rabbit Polyclonal anti-MBD1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MBD1 (RC234662, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MBD1 ra. Positive lysates LY414370 (100ug) and LC414370 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~2000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human MBD1
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 3.76 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.8 kDa
• Gene Name: methyl-CpG binding domain protein 1
• Database Link:
  NP_056669
  Entrez Gene 4152 Human
  UniProt ID Q9UIS9
• Background: The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]
• Synonyms: CXXC3; PCM1; RFT

Reference Data

• Protein Families: Druggable Genome, Transcription Factors