NUP214 Rabbit Polyclonal Antibody

CAT#: TA890139

Rabbit Polyclonal anti-NUP214 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUP214 (RC206891, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUP214 rabbit polyclonal antibody.

• 

  Western blot analysis of extracts (35ug) from different cell lines and tissues by using anti-NUP214 rabbit polyclonal antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~2000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human NUP214
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 1.68 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 213.4 kDa
• Gene Name: nucleoporin 214kDa
• Database Link:
  NP_005076
  Entrez Gene 227720 MouseEntrez Gene 8021 Human
  UniProt ID P35658
• Background: The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
• Synonyms: CAIN; CAN