AMPK beta 1 (PRKAB1) Rabbit Polyclonal Antibody

CAT#: TA890141S

Rabbit Polyclonal anti-PRKAB1 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAB1 (RC203911, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAB. Positive lysates LY416773 (100ug) and LC416773 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines and tissues by using anti-PRKAB1 rabbit polyclonal antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human PRKAB1
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 3 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.2 kDa
• Gene Name: protein kinase AMP-activated non-catalytic subunit beta 1
• Database Link:
  NP_006244
  Entrez Gene 19079 MouseEntrez Gene 83803 RatEntrez Gene 5564 Human
  UniProt ID Q9Y478
• Background: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). [UniProtKB/Swiss-Prot Function]
• Synonyms: AMPK; HAMPKb

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway