RAD52 Rabbit Polyclonal Antibody

CAT#: TA890144S

Rabbit Polyclonal anti-RAD52 Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD52 (RC222194, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD52. Positive lysates LY408748 (100ug) and LC408748 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell lines and tissues by using anti-RAD52 rabbit polyclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RAD52-Knockout HeLa cells (KO, Cat# LC830600) were separated by SDS-PAGE and immunoblotted with anti-RAD52 rabbit polyclonal antibody TA890144 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human RAD52
• Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Concentration: 2.54 mg/ml
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: RAD52 homolog, DNA repair protein
• Database Link:
  NP_602296
  Entrez Gene 19365 MouseEntrez Gene 297561 RatEntrez Gene 5893 Human
  UniProt ID P43351
• Synonyms: RP11-359B12.2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination