PAX5 Rabbit Polyclonal Antibody

CAT#: TA899004S

PAX5 Rabbit Polyclonal Antibody

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX5 cDNA (Cat# RC222785, Right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX5(Cat# TA899004).(1:500)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA 1:5000-10000, WB 1:500
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This antibody is raised against a recombinant protein of PAX5 expressed in HEK293 cells
• Formulation: Tissue culture buffer containing 1% BSA and 0.02% Sodium azide
• Concentration: Lot dependent; please refer to CoA along with shipment
• Purification: Purified from the immunized serum by affinity chromatography (Protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: paired box 5
• Database Link:
  NP_057953
  Entrez Gene 5079 Human
  UniProt ID Q02548
• Background: This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: ALL3; BSAP

Reference Data

• Protein Families: Transcription Factors