Cytokeratin 19 (KRT19) Mouse Monoclonal Antibody [Clone ID: UMAB2]

CAT#: UM500002

KRT19/CK19 mouse monoclonal antibody,clone UMAB2

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CK19 monoclonal antibody (Clone UMAB2) at 1:500.

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  HEK293T were transfected with 55 different plasmids of CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 antibody. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative (1:500).

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  Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CK19 monoclonal antibody (clone UMAB2, 1:500)

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  Immunohistochemical staining of paraffin-embedded breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-KRT19 mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human liver tissue using anti-KRT19 mouse monoclonal antibody. Anti-KRT19 clone UMAB2 was diluted 1:100; used heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min to produce strong staining on the bile duct and no staining in the hepatocytes.

• Immunohistochemical staining of paraffin-embedded lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human pancreas tissue using anti-KRT19mouse monoclonal antibody. Clone UMAB2 was diluted 1:100; used heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min to produce strong staining on the exocrine glandular cells and no staining in the Islets of Langerhans

• Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-KRT19mouse monoclonal antibody. (Clone UMAB2, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunofluorescent staining of MCF7 cells using anti-CK19 mouse monoclonal antibody (UM500002, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

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Specifications

Product Data

• Clone Name: UMAB2
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens keratin 19 (KRT19), mRNA.
• Database Link:
  NP_002267
  Entrez Gene 3880 Human
  UniProt ID P08727
• Background: Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.
• Synonyms: CK19; K1CS; K19