BubR1 (BUB1B) Mouse Monoclonal Antibody [Clone ID: UMAB7]

CAT#: UM500007CF

Carrier-free (BSA/glycerol-free) anti-BUB1B mouse monoclonal antibody, clone UMAB7

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody (Clone UMAB7) at 1:500.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-BUB1Bmouse monoclonal antibody. (Clone UMAB7, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human endometrial carcinoma using anti-BUB1B a mouse monoclonal antibody. Heat-induced epitope retrieval by ACCEL (pH8.7) in pressure chamber/cooker at 110°C for 3min, UM500007 clone UMAB7 at 1:250. Images shows tumor cells with strong cytoplasmic staining.

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  Immunofluorescent staining of A549 cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

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  Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (UM500007, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

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Specifications

Product Data

• Clone Name: UMAB7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100, IF 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Protein expressed in 293T cell transfected with human BUB1B expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 119.5 kDa
• Gene Name: Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.
• Database Link:
  NP_001202
  Entrez Gene 100423079 MonkeyEntrez Gene 701 Human
  UniProt ID O60566
• Background: This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
• Synonyms: Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle