CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: UMAB30]

CAT#: UM500030CF

Carrier-free (BSA/glycerol-free) PECAM1 mouse monoclonal antibody,clone UMAB30

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1 mouse monoclonal antibody.1:500`

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  Western Blot analysis of MOLT-4 cell lysate (35ug) by using anti-PECAM1 monoclonal antibody (Clone UMAB30)

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECAM1 monoclonal antibody (Clone UMAB30) at 1:500.

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  Western blot analysis of extracts (35ug) from 10 different human tissues and Jurkat cell line by using anti-PECAM1 monoclonal antibody. 1:250`

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  Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-PECAM1 monoclonal antibody (clone UMAB30, 1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-PECAM1 mouse monoclonal antibody. (Clone UMAB30, Dilution 1:100; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PECAM1 mouse monoclonal antibody. (Clone UMAB30, Dilution 1:100; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-PECAM1 mouse monoclonal antibody. (Clone UMAB30, Dilution 1:100; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-PECAM1 mouse monoclonal antibody. (Clone UMAB30, Dilution 1:100; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-PECAM1 mouse monoclonal antibody. (Clone UMAB30, Dilution 1:100; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human placenta using anti-PECAM clone UMAB30 mouse monoclonal antibody (UM500030) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the endothelia cells and weak staining in the trophoblast.

• Immunohistochemical staining of paraffin-embedded human skin using anti-PECAM clone UMAB30 mouse monoclonal antibody (UM500030) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic and membraneous staining is seen in the vasular endothelia cells.

• Living HEK293T cells transfected with either RC208654 plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody (UM500030) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PECAM1 mouse monoclonal antibody (Clone UMAB30). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-PECAM1 (Clone UMAB30) very specifically recognizes PECAM1 antigen on OriGene protein microarray chip.

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Specifications

Product Data

• Clone Name: UMAB30
• Applications: 10k-ChIP, FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full lenth human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.4 kDa
• Gene Name: Homo sapiens platelet and endothelial cell adhesion molecule 1 (PECAM1)
• Database Link:
  NP_000433
  Entrez Gene 5175 Human
  UniProt ID P16284
• Background: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May
• Synonyms: CD31; EndoCAM; endoCAM; GPIIA; PECA1; PECAM-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration