CD1C Mouse Monoclonal Antibody [Clone ID: UMAB46]

CAT#: UM500042CF

Carrier-free (BSA/glycerol-free) CD1C mouse monoclonal antibody,clone UMAB46

Product Images

Western Blot analysis of MOLT-4 cell lysate (35ug) by using anti-CD1C monoclonal antibody (Clone UMAB46)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CD1C mouse monoclonal antibody. (UM500042; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded human tonsil with mouse anti-CD1c clone UMAB46 1:200 dilution of 1mg/mL using HIER citrate pressure chamber. Shown here is a composit of 10x and 20x Imaging of tonsil with expression cytoplasmic and membraneous staining pattern.

• 

  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD1C mouse monoclonal antibody (UM500042). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Specifications

Product Data

• Clone Name: UMAB46
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD1C(NP_001756) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: Homo sapiens CD1c molecule (CD1C), mRNA.
• Database Link:
  NP_001756
  Entrez Gene 911 Human
  UniProt ID P29017
• Background: This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq, Jul
• Synonyms: BDCA1; CD1; CD1A; R7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage