p53 (TP53) Mouse Monoclonal Antibody [Clone ID: UMAB62]

CAT#: UM500053

Purified P53 (TP53) mouse monoclonal antibody, clone UMAB62

Product Images

Western Blot analysis of extracts (35ug) from 11 different cell lines by using anti-TP53 monoclonal antibody (Clone UMAB62)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TP53 mouse monoclonal antibody. (UM500053; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human normal colon tissue and colorectal cancer tissue using anti-TP53 mouse monoclonal antibody. (UM500053; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human normal endometrium tissue and endometrium adenocarcinoma tissue using anti-TP53 mouse monoclonal antibody. (UM500053; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunofluorescent staining of COS7 cells using TP53 mouse monoclonal antibody (UM500053, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

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  Immunofluorescent staining of HT-29 cells using TP53 mouse monoclonal antibody (UM500053, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TP53 mouse monoclonal antibody (UM500053). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB62
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:50~100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.5 kDa
• Gene Name: Homo sapiens tumor protein p53 (TP53), transcript variant 1, mRNA.
• Database Link:
  NP_000537
  Entrez Gene 7157 Human
  UniProt ID P04637
• Background: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]
• Synonyms: BCC7; LFS1; P53; TRP53

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway