B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: UMAB65]

CAT#: UM500055CF

Carrier-free (BSA/glycerol-free) CD80 mouse monoclonal antibody, clone UMAB65

Product Images

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-CD80 mouse monoclonal antibody. (UM500055; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-CD80 mouse monoclonal antibody (UM500055). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Specifications

Product Data

• Clone Name: UMAB65
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.3 kDa
• Gene Name: Homo sapiens CD80 molecule (CD80), mRNA.
• Database Link:
  NP_005182
  Entrez Gene 941 Human
  UniProt ID P33681
• Background: The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
• Synonyms: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis