LAT2 (SLC7A8) Mouse Monoclonal Antibody [Clone ID: UMAB70]

CAT#: UM500058CF

Carrier-free (BSA/glycerol-free) SLC7A8 mouse monoclonal antibody,clone UMAB70

Product Images

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human normal colon tissue and colorectal cancer tissue using anti-SLC7A8 mouse monoclonal antibody. (UM500058; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human normal kidney tissue and kidney carcinoma tissue using anti-SLC7A8 mouse monoclonal antibody. (UM500058; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human normal pancreas tissue and pancreatic cancer tissue using anti-SLC7A8 mouse monoclonal antibody. (UM500058; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunofluorescent staining of HeLa cells using anti-SLC7A8 mouse monoclonal antibody (UM500058, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SLC7A8 mouse monoclonal antibody (UM500058). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB70
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLC7A8(NP_036376) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.2 kDa
• Gene Name: solute carrier family 7 member 8
• Database Link:
  NP_036376
  Entrez Gene 50934 MouseEntrez Gene 84551 RatEntrez Gene 23428 Human
  UniProt ID Q9UHI5
• Synonyms: LAT2; LPI-PC1

Reference Data

• Protein Families: Druggable Genome, Transmembrane