CD64 (FCGR1A) Mouse Monoclonal Antibody [Clone ID: UMAB74]

CAT#: UM500062CF

Carrier-free (BSA/glycerol-free) FCGR1A mouse monoclonal antibody,clone UMAB74

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR1A (RC207487, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR1A (1:2000).

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  Western blot analysis of extracts (35ug) from 1 cell line and 1 tissue lysates by using anti-FCGR1A monoclonal antibody (1:500).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunofluorescent staining of HeLa cells using anti-FCGR1A mouse monoclonal antibody (UM500062, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

• Flow cytometric analysis of living 293T cells transfected with FCGR1A overexpression plasmid (RC207487), Red)/empty vector (PS100001, Blue) using anti-FCGR1A antibody (UM500062). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FCGR1A mouse monoclonal antibody (UM500062). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB74
• Applications: 10k-ChIP, FC, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FCGR1A(NP_000557) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.5 kDa
• Gene Name: Homo sapiens Fc fragment of IgG receptor Ia (FCGR1A), transcript variant 2, mRNA.
• Database Link:
  NP_000557
  Entrez Gene 2209 Human
  UniProt ID P12314
• Background: This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
• Synonyms: CD64; CD64A; FCRI; IGFR1

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Fc gamma R-mediated phagocytosis, Hematopoietic cell lineage, Systemic lupus erythematosus