PFDN3 (VBP1) Mouse Monoclonal Antibody [Clone ID: UMAB75]

CAT#: UM500063CF

Carrier-free (BSA/glycerol-free) VBP1 mouse monoclonal antibody,clone UMAB75

Product Images

Western Blot analysis of HT-29, BT-549 and MOLT-4 cell lysates (35ug) by using anti-VBP1 monoclonal antibody (Clone UMAB75)

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-VBP1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Western blot of mouse tissue lysates (20ug) from Ovary. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-VBP1 mouse monoclonal antibody. (UM500063; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VBP1 mouse monoclonal antibody. (UM500063; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-VBP1 mouse monoclonal antibody. (UM500063; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-VBP1 mouse monoclonal antibody. (UM500063; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-VBP1 mouse monoclonal antibody. (UM500063; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VBP1 mouse monoclonal antibody. (UM500063; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunofluorescent staining of MDA-MB-231 cells using anti-VBP1 mouse monoclonal antibody (UM500063, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-VBP1 mouse monoclonal antibody (UM500063). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB75
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VBP1(NP_003363) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.4 kDa
• Gene Name: Homo sapiens VHL binding protein 1 (VBP1), transcript variant 1, mRNA.
• Database Link:
  NP_003363
  Entrez Gene 22327 MouseEntrez Gene 7411 Human
  UniProt ID P61758
• Background: The protein encoded by this gene interacts with the Von Hippel-Lindau protein to form an intracellular complex. Because it functions as a chaperone protein, it is suspected that it may play a role in the transport of the Von Hippel-Lindau protein from the perinuclear granules to the nucleus or cytoplasm. [provided by RefSeq, Jul 2008]
• Synonyms: HIBBJ46; PFD3; PFDN3; VBP-1

Reference Data

• Protein Families: Druggable Genome