EGFR Mouse Monoclonal Antibody [Clone ID: UMAB95]

CAT#: UM500070CF

Carrier-free (BSA/glycerol-free) EGFR mouse monoclonal antibody,clone UMAB95

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EGFR monoclonal antibody (Clone UMAB95).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and EGFR-Knockout HeLa cells (KO, Cat# LC831281) were separated by SDS-PAGE and immunoblotted with anti-EGFR monoclonal antibody UM500070 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human placenta with mouse anti-EGFR clone UMAB95 1:200 dilution of 400ug/mL using HIER citrate pH6.o pressure chamber. Placenta known to express high levels in the cytoplasm and membrane trophoblastic cells

• Immunohistochemical staining of paraffin-embedded human lung cancer with mouse anti-EGFR clone UMAB95 1:200 dilution of 400ug/mL using HIER citrate pH6.0 pressure chamber. Expression in lung cancer cells are cytoplasmic and membraneous

• Immunofluorescent staining of HeLa cells using anti-EGFR mouse monoclonal antibody (UM500070, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-EGFR mouse monoclonal antibody (UM500070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB95
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EGFR(NP_958440) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.4 kDa
• Gene Name: Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 3, mRNA.
• Database Link:
  NP_958440
  Entrez Gene 13649 MouseEntrez Gene 24329 RatEntrez Gene 613027 MonkeyEntrez Gene 1956 Human
  UniProt ID P00533
• Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Synonyms: ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Secreted Protein, Stem cell relevant signaling - JAK/STAT signaling pathway, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Dorso-ventral axis formation, Endocytosis, Endometrial cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton