CD19 Mouse Monoclonal Antibody [Clone ID: UMAB103]

CAT#: UM500071

CD19 mouse monoclonal antibody, clone UMAB103

Product Images

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD19 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Western blot of cell lysates (35ug) from Jurkat. Diluation: 1:500

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD19 clone UMAB103 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500071 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >40 % of non germinal center cells of tonsil and >90% of the germinal center cells. No staining was seen in the squamous epithelia cells.

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  Flow Cytometric analysis of viable B-Cell Lymphoma cell line (Raji) using anti-CD19 antibody (UM500071-red histogram), compared to isotype control antibody (anti-DDK TA180144- green histogram), and no antibody control PBS (blue histogram). (CD19 titer 1:20)

• Flow Cytometric analysis of viable human peripheral blood mononuclear cells (PBMCs) using anti-human CD19 antibody (UM500071-red histogram), compared to isotype control antibody (anti-DDK TA180144 - green histogram). (CD19 titer 1:20)

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Specifications

Product Data

• Clone Name: UMAB103
• Applications: FC, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD19(NP_001761) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.9 kDa
• Gene Name: Homo sapiens CD19 molecule (CD19), transcript variant 2, mRNA.
• Database Link:
  NP_001761
  Entrez Gene 930 Human
  UniProt ID P15391
• Background: Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
• Synonyms: B4; CVID3
• Note: This antibody is the same clone with TA506236 which is recently upgraded to be an UltraMAB.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency