ZNF447 (ZSCAN18) Mouse Monoclonal Antibody [Clone ID: UMAB114]

CAT#: UM500081CF

Carrier-free (BSA/glycerol-free) ZSCAN18 mouse monoclonal antibody,clone UMAB114

Product Images

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZSCAN18mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ZSCAN18 mouse monoclonal antibody. (UM500081; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ZSCAN18 mouse monoclonal antibody (UM500081). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB114
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZSCAN18(NP_076415) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.6 kDa
• Gene Name: Homo sapiens zinc finger and SCAN domain containing 18 (ZSCAN18), transcript variant 3, mRNA.
• Database Link:
  NP_076415
  Entrez Gene 65982 Human
  UniProt ID Q8TBC5
• Synonyms: ZNF447

Reference Data

• Protein Families: Transcription Factors