IDO1 Mouse Monoclonal Antibody [Clone ID: UMAB126]

CAT#: UM500091CF

Carrier-free (BSA/glycerol-free) IDO1 mouse monoclonal antibody,clone UMAB126

Product Images

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

• 

  Western blot of mouse tissue lysates (20ug) from Brian. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-IDO1 mouse monoclonal antibody. (UM500091; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded human placenta using anti-IDO1 clone UMAB126 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500091 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The placenta shows strong membrane and cytoplasmic in the endothelial cells.

• Immunohistochemical staining of paraffin-embedded human tonsil using anti-IDO1 clone UMAB126 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500091 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tonsil shows strong membrane and cytoplasmic in the germinal center and rare strong nuclear, membrane, and cytoplasmic staining in the non germinal centers.

• Immunohistochemical staining of paraffin-embedded human spleen using anti-IDO1 clone UMAB126 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM500091 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The spleen shows very few cells staining in the red pulp with strong nuclear, membrane, and cytoplasmic staining.

• Immunofluorescent staining of MCF-7 cells using anti-IDO1 mouse monoclonal antibody (UM500091, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-IDO1 mouse monoclonal antibody (UM500091). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Show all 10 images

Specifications

Product Data

• Clone Name: UMAB126
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100~200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IDO1(NP_002155) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.1 kDa
• Gene Name: Homo sapiens indoleamine 2,3-dioxygenase 1 (IDO1), mRNA.
• Database Link:
  NP_002155
  Entrez Gene 3620 Human
  UniProt ID P14902
• Background: This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. [provided by RefSeq, Feb 2011]
• Synonyms: IDO; IDO-1; INDO

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Tryptophan metabolism