EPCAM Mouse Monoclonal Antibody [Clone ID: UMAB131]

CAT#: UM500096CF

Carrier-free (BSA/glycerol-free) EPCAM mouse monoclonal antibody,clone UMAB131

Product Images

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).

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  Western blot analysis of LOVO cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

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  Western blot analysis of COLO205 cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-EPCAM monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Western blot analysis of KM12 cell lysate (35ug) by using anti-EPCAM monoclonal antibody.

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPCAM mouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EPCAM mouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-EPCAM mouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-EPCAMmouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-EPCAM mouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPCAM mouse monoclonal antibody. (UM500096; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Flow cytometric analysis of live HT29 cells, using anti-EPCAM antibody (UM500096, clone UMAB131 at 1:100, Red), compared to IgG1 isotype control (Black).

• Flow cytometric analysis of live OVCAR-4 cells, using anti-EPCAM antibody (UM500096, clone UMAB131 at 1:100, Red), compared to IgG1 isotype control (Black).

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Specifications

Product Data

• Clone Name: UMAB131
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens epithelial cell adhesion molecule (EPCAM), mRNA.
• Database Link:
  NP_002345
  Entrez Gene 4072 Human
  UniProt ID P16422
• Background: This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
• Synonyms: 4; DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane