BID Mouse Monoclonal Antibody [Clone ID: UMAB139]

CAT#: UM500101CF

Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone UMAB139

Product Images

Western blot analysis of HEK293 cell lysate (35ug) by using anti-BID monoclonal antibody.

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  Western blot analysis of COLO205 cell lysate (35ug) by using anti-BID monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BID monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Western blot analysis of DU145 cell lysate (35ug) by using anti-BID monoclonal antibody UM500101

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded human kidney carcinoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.

• Immunohistochemical staining of paraffin-embedded human melanoma using BID clone UMAB139, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM500101 requires heat-induced epitope retrieval with Citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image shows strong nuclear, membranous, and cytoplasmic staining in the tumor cells.

• Immunohistochemical staining of paraffin-embedded of 3 human colon cancers using anti-BID clone UMAB139 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500101 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The composit image of 3 colon cancers labeled A, B, and C shows strong membranous and cytoplasmic staining in >75 % of tumor cells and image A and B shows <10% nuclear stain.

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Specifications

Product Data

• Clone Name: UMAB139
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2, mRNA.
• Database Link:
  NP_001187
  Entrez Gene 637 Human
  UniProt ID P55957
• Background: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
• Synonyms: FP497

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Natural killer cell mediated cytotoxicity, p53 signaling pathway, Pathways in cancer, Viral myocarditis