GFAP Mouse Monoclonal Antibody [Clone ID: UMAB129]

CAT#: UM570094

GFAP mouse monoclonal antibody, clone UMAB129

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP (1:500).

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  Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

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  Western blot analysis of extracts (35ug) from 1 cell line lysate and 1 tissue lysate by using anti-GFAP monoclonal antibody (1:500).

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  Western blot analysis of DU145 cell lysate (35ug) by using anti-GFAP monoclonal antibody.

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

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  Western blot of mouse tissue lysates (20ug) from Brain. Diluation: 1:500.

• Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-GFAP mouse monoclonal antibody. (UM500094; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

• Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-GFAP mouse monoclonal antibody. (UM500094; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

• Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-GFAP mouse monoclonal antibody. (UM500094; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

• Confocal immunofluoresce image of primary rat neurons labeled with anti-GFAP mouse monoclonal antibody (UM500094, green, 1:100) and with DAPI (blue) for nuclear. Scale bar, 10um.

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Specifications

Product Data

• Clone Name: UMAB129
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GFAP (NP_002046) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1, mRNA.
• Database Link:
  NP_002046
  Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human
  UniProt ID P14136
• Background: This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
• Synonyms: ALXDRD

Reference Data

• Protein Families: ES Cell Differentiation/IPS