MADCAM1 Mouse Monoclonal Antibody [Clone ID: UMAB158]

CAT#: UM570105

MADCAM1 mouse monoclonal antibody,clone UMAB158

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1 (1:2000).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM1 (RC219060, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MADCAM1 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MADCAM1 mouse monoclonal antibody. (UM500105, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MADCAM1 mouse monoclonal antibody. (UM500105, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MADCAM1 mouse monoclonal antibody. (UM500105, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MADCAM1 mouse monoclonal antibody. (UM500105, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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Specifications

Product Data

• Clone Name: UMAB158
• Applications: IHC
• Recommended Dilution: WB 1:2000, IHC 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MADCAM1(NP_570116) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mRNA.
• Database Link:
  NP_570116
  Entrez Gene 8174 Human
  UniProt ID Q13477
• Background: The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: MACAM1

Reference Data

• Protein Pathways: Cell adhesion molecules (CAMs)