CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: UMAB37]

CAT#: UM800001

CD20 mouse monoclonal antibody,clone UMAB37

Product Images

Immunohistochemical staining of paraffin-embedded lymph node tissue using anti-CD20 mouse monoclonal antibody. (Clone UMAB37, Dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800001 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The spleen shows strong membrane and cytoplasmic staining in red and white pulp cells.

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  Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800001 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tonsil shows strong membrane and cytoplasmic staining in the germinal center of the tonsil and some poitive cells in the non-germinal center.

Specifications

Product Data

• Clone Name: UMAB37
• Applications: IHC
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.9 kDa
• Gene Name: Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3, mRNA.
• Database Link:
  NP_068769
  Entrez Gene 931 Human
  UniProt ID P11836
• Background: This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]
• Synonyms: B1; Bp35; CD20; CVID5; LEU-16; MS4A2; S7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage