CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: UMAB38]

CAT#: UM800002CF

Carrier-free (BSA/glycerol-free) CD20 mouse monoclonal antibody,clone UMAB38

Product Images

Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CD20 monoclonal antibody (clone UMAB38, 1:500)

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  Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800002 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The spleen shows strong membrane and cytoplasmic staining in red and white pulp cells.

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  Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800002 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tonsil shows strong membrane and cytoplasmic staining in the germinal center of the tonsil and some poitive cells in the non-germinal center.

Specifications

Product Data

• Clone Name: UMAB38
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.9 kDa
• Gene Name: Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3, mRNA.
• Database Link:
  NP_068769
  Entrez Gene 931 Human
  UniProt ID P11836
• Background: This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]
• Synonyms: B1; Bp35; CD20; CVID5; FMC7; LEU-16; MS4A2; S7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage