CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: UMAB39]

CAT#: UM800003CF

Carrier-free (BSA/glycerol-free) CD20 mouse monoclonal antibody,clone UMAB39

Product Images

Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 clone UMAB39 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800003 requires heat-induced epitope retrieval withCitrate pH6.0 for 3minutes at110C in pressure chamber. The image shows the cells in the white pulp have strong cytoplasmic and membraneous staining.

Specifications

Product Data

• Clone Name: UMAB39
• Applications: IHC
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.9 kDa
• Gene Name: Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3, mRNA.
• Database Link:
  NP_068769
  Entrez Gene 931 Human
  UniProt ID P11836
• Background: This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq]
• Synonyms: B1; Bp35; CD20; CVID5; LEU-16; MS4A2; S7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage