c-Jun (JUN) Mouse Monoclonal Antibody [Clone ID: UMAB49]

CAT#: UM800005CF

Carrier-free (BSA/glycerol-free) JUN mouse monoclonal antibody,clone UMAB49

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-JUN monoclonal antibody (Clone UMAB49).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-JUN mouse monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-JUN mouse monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-JUN monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-JUN mouse monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-JUN mouse monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunofluorescent staining of HeLa cells using JUN mouse monoclonal antibody (UM800005, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

• Immunofluorescent staining of HeLa cells using anti-JUN mouse monoclonal antibody (UM800005, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-JUN mouse monoclonal antibody (UM800005). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB49
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:50~100, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human JUN (NP_002219) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.5 kDa
• Gene Name: Homo sapiens Jun proto-oncogene, AP-1 transcription factor subunit (JUN), mRNA.
• Database Link:
  NP_002219
  Entrez Gene 16476 MouseEntrez Gene 24516 RatEntrez Gene 609429 DogEntrez Gene 3725 Human
  UniProt ID P05412
• Background: This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
• Synonyms: AP-1; AP1; c-Jun

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Renal cell carcinoma, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Wnt signaling pathway