CD20 (MS4A1) Mouse Monoclonal Antibody [Clone ID: UMAB58]

CAT#: UM800009CF

Carrier-free (BSA/glycerol-free) Purified CD20 (MS4A1) mouse monoclonal antibody,clone UMAB58

Product Images

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MS4A1 mouse monoclonal antibody (UM800009). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.

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  Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 clone UMAB58 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800009 requires heat-induced epitope retrieval withCitrate pH6.0 for 3minutes at110C in pressure chamber. The image shows the cells in the white pulp have strong cytoplasmic and membraneous staining.

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  Immunofluorescent staining of Jurkat cells using anti-CD20 mouse monoclonal antibody (UM800009, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.

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Specifications

Product Data

• Clone Name: UMAB58
• Applications: 10k-ChIP, IF, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.9 kDa
• Gene Name: Homo sapiens membrane spanning 4-domains A1 (MS4A1), transcript variant 3, mRNA.
• Database Link:
  NP_068769
  Entrez Gene 931 Human
  UniProt ID P11836
• Background: This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul
• Synonyms: B1; Bp35; CD20; CVID5; LEU-16; MS4A2; S7

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage