HE4 (WFDC2) Mouse Monoclonal Antibody [Clone ID: UMAB87]

CAT#: UM800018CF

Carrier-free (BSA/glycerol-free) Purified HE4 (WFDC2) mouse monoclonal antibody,clone UMAB87

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WFDC2 mouse monoclonal antibody. (UM800018; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-WFDC2 mouse monoclonal antibody. (UM800018; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded 3 human ovarian cancer using anti-HE4 clone UMAB87 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.

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  Immunohistochemical staining of paraffin-embedded 3 human endometrial cancer using anti-HE4 clone UMAB87 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tumor cells show membrane and cytoplasmic staining.

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  Immunohistochemical staining of paraffin-embedded human colon using anti-HE4 clone UMAB87 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800018 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The cells show membrane and cytoplasmic staining.

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  OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WFDC2 mouse monoclonal antibody (UM800018). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB87
• Applications: 10k-ChIP, IHC
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 31-124 of human WFDC2 (NP_006094) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 10 kDa
• Gene Name: Homo sapiens WAP four-disulfide core domain 2 (WFDC2), mRNA.
• Database Link:
  NP_006094
  Entrez Gene 10406 Human
  UniProt ID Q14508
• Background: This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]
• Synonyms: dJ461P17.6; EDDM4; HE4; WAP5

Reference Data

• Protein Families: Secreted Protein, Transmembrane