FOXP1 Mouse Monoclonal Antibody [Clone ID: UMAB89]

CAT#: UM800020CF

Carrier-free (BSA/glycerol-free) FOXP1 mouse monoclonal antibody,clone UMAB89

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FOXP1 monoclonal antibody (Clone UMAB89).

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-FOXP1 mouse monoclonal antibody. (UM800020; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FOXP1 mouse monoclonal antibody. (UM800020; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-FOXP1 mouse monoclonal antibody. (UM800020; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human lung cancer using anti-FOXP1 clone UMAB89 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800020 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The panel A shows negative tumor cells of lung carcinoma and panel B strong nuclear staining in the tumor cells.

• OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOXP1 mouse monoclonal antibody (UM800020). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

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Specifications

Product Data

• Clone Name: UMAB89
• Applications: 10k-ChIP, IHC, WB
• Recommended Dilution: IHC 1:100, IF 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOXP1 (NP_116071) produced in SF9 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.1 kDa
• Gene Name: Homo sapiens forkhead box P1 (FOXP1), transcript variant 1, mRNA.
• Database Link:
  NP_116071
  Entrez Gene 108655 MouseEntrez Gene 297480 RatEntrez Gene 27086 Human
  UniProt ID Q9H334
• Background: This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul
• Synonyms: 12CC4; hFKH1B; HSPC215; MFH; QRF1

Reference Data

• Protein Families: Transcription Factors