CD23 (FCER2) Mouse Monoclonal Antibody [Clone ID: UMAB101]

CAT#: UM800031

CD23 (FCER2) mouse monoclonal antibody,clone UMAB101

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCER2 (RC204335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCER2 (1:2000).

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  Immunohistochemical staining of paraffin-embedded human tonsil using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:400 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 tonsils which show strong membranous and cytoplasmic staining however each tonsil has a unique expression pattern in the inner and outer germinal center.

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  Immunohistochemical staining of paraffin-embedded human spleen using FCER2 (CD23) clone UMAB101, mouse monoclonal antibody at 1:100 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800031 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image is a composite of 3 spleens which show strong membranous and cytoplasmic staining white pulp.

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  Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue within the normal limits using anti-CD23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800031) (1:500)

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  Immunohistochemical staining of paraffin-embedded mouse spleen tissue within the normal limits using anti-FCER2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800031) (1:500)

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  Immunofluorescent staining of Jurkat cells using anti-CD23 mouse monoclonal antibody (UM800031, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

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Specifications

Product Data

• Clone Name: UMAB101
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 48-321 of human FCER2 (NP_001993) produced in SF9 Cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5~1.0 mg/ml (Lot Dependent)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.3 kDa
• Gene Name: Homo sapiens Fc fragment of IgE receptor II (FCER2), transcript variant 1, mRNA.
• Database Link:
  NP_001993
  Entrez Gene 2208 Human
  UniProt ID P06734
• Background: The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul
• Synonyms: BLAST-2; CD23; CD23A; CLEC4J; FCE2; IGEBF

Reference Data

• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Hematopoietic cell lineage