Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: UMAB107]

CAT#: UM800033CF

Carrier-free (BSA/glycerol-free) Purified KI67 (MKI67) mouse monoclonal antibody,clone UMAB107

Product Images

Western Blot analysis of HT-29 and NCI-H322M cell lysates (35ug) by using anti-KI67 monoclonal antibody (Clone UMAB107)

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  Western Blot analysis of MOLT-4 and MDA-MB231 cell lysates (35ug) by using anti-KI67 monoclonal antibody (Clone UMAB107)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded normal human tonsil tissue using anti-MKI67 mouse monoclonal antibody. (UM800033 at 1ug/ml for 30 min at RT; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 20min; )

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  Immunohistochemical staining of paraffin-embedded mouse colon tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded of 3 human colon cancer using anti-Ki67 clone UMAB107 mouse monoclonal antibody at 1:1000 dilution of 0.5mg/mL and detection with Polink2 Broad HRP DAB. UM800033 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human colon cancer all show strong nuclear staining in the tumor cells.

• Immunohistochemical staining of paraffin-embedded of 3 human lung cancer using anti-Ki67 clone UMAB107 mouse monoclonal antibody at 1:1000 dilution of 0.5mg/mL and detection with Polink2 Broad HRP DAB. UM800033 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human lung cancer all show strong nuclear staining in the tumor cells.

• Immunofluorescent staining of MDA-MB-231 cells using anti-KI67 mouse monoclonal antibody (UM800033, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

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Specifications

Product Data

• Clone Name: UMAB107
• Applications: IF, IHC, WB
• Recommended Dilution: IHC: 1:300
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens marker of proliferation Ki-67 (MKI67), transcript variant 1, mRNA.
• Database Link:
  NP_002408
  Entrez Gene 4288 Human
  UniProt ID P46013
• Background: This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
• Synonyms: KIA; MIB-; MIB-1; PPP1R105

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS